Influence of Thymine Starvation on the Integrity of Episomal and Chromosomal Deoxyribonucleic Acids in Escherichia coli CR34 (λ ind − )-Reference-Cited by-同舟云学术

Influence of Thymine Starvation on the Integrity of Episomal and Chromosomal Deoxyribonucleic Acids in Escherichia coli CR34 (λ ind − )

Published:1973-11 Issue:2 Volume:116 Page:1067-1070
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Shuster Robert C.¹

Affiliation:

1. Department of Biochemistry, Emory University, Atlanta, Georgia 30322

Abstract

The effect of thymine deprivation on the integrity of phage λ, sex factor, and chromosomal deoxyribonucleic acid (DNA) in Escherichia coli CR34 (λ ind − ) was examined by sedimenting cell lysates through alkaline sucrose gradients. Both sex factor and chromosomal DNAs showed evidence of being degraded during the starvation period. In contrast, no loss of closed circular λ DNA was observed.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jb.116.2.1067-1070.1973

Reference20 articles.

1. Influence of thymine starvation on the integrity of deoxyribonucleic acid in Escherichia coli;Baker M. L.;J. Bacteriol.,1971

2. Protein synthesis and deoxyribonucleic acid membrane attachment during thymineless death in Escherichia coli;Dankberg F.;J. Bacteriol.,1973

3. Technique for starvation of Escherichia coli of thymine;Freifelder D.;J. Bacteriol.,1965

4. Single strand breaks in bacterial DNA associated with thymine starvation;Freifelder D.;J. Mol. Biol.,1969

5. Studies on Escherichia coli sex factors II. Some physical properties of F'Lac and F DNA;Freifelder D. R.;J. Mol. Biol.,1968

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