Affiliation:
1. Department of Biology, The University of Texas M. D. Anderson Hospital and Tumor Institute, Houston, Texas 77025
Abstract
The influence of thymine starvation on the single-strand molecular weight of deoxyribonucleic acid (DNA) from
Escherichia coli
was determined by sedimentation through gradients of alkaline sucrose. Growth of cells for as long as 150 min in thymineless medium did not significantly reduce the molecular weight below the control value of 2.4 ± 0.3 × 10
8
daltons. Incubation of cells in thymineless medium after exposure to 500 ergs/mm
2
of ultraviolet light or 20 krad of
137
Cs gamma rays did not appear to block the rejoining of single-strand breaks associated with irradiation. Thus, DNA repair enzymes, presumably including DNA ligase, are not significantly inhibited by thymine starvation.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
19 articles.
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