Integron-Associated DfrB4, a Previously Uncharacterized Member of the Trimethoprim-Resistant Dihydrofolate Reductase B Family, Is a Clinically Identified Emergent Source of Antibiotic Resistance-Reference-Cited by-同舟云学术

Integron-Associated DfrB4, a Previously Uncharacterized Member of the Trimethoprim-Resistant Dihydrofolate Reductase B Family, Is a Clinically Identified Emergent Source of Antibiotic Resistance

Published:2017-05 Issue:5 Volume:61 Page:
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Toulouse Jacynthe L.¹²³,Edens Thaddeus J.⁴,Alejaldre Lorea¹²³,Manges Amee R.⁵,Pelletier Joelle N.¹²³⁶

Affiliation:

1. Département de biochimie, Université de Montréal, C.P. 6128,Succursale Centre-ville, Montréal, Québec, Canada

2. CGCC, Center in Green Chemistry and Catalysis, Montréal, Québec, Canada

3. PROTEO, Québec Network for Protein Function, Engineering and Applications, Québec, Québec, Canada

4. Devil's Staircase Consulting, North Vancouver, British Columbia, Canada

5. School of Population and Public Health, University of British Columbia, Vancouver, British Columbia, Canada

6. Département de chimie, Université de Montréal, C.P. 6128, Succursale Centre-ville, Montréal, Québec, Canada

Abstract

ABSTRACT Whole-genome sequencing of trimethoprim-resistant Escherichia coli clinical isolates identified a member of the trimethoprim-resistant type II dihydrofolate reductase gene family ( dfrB ). The dfrB4 gene was located within a class I integron flanked by multiple resistance genes. This arrangement was previously reported in a 130.6-kb multiresistance plasmid. The DfrB4 protein conferred a >2,000-fold increased trimethoprim resistance on overexpression in E. coli . Our results are consistent with the finding that dfrB4 contributes to clinical trimethoprim resistance.

Funder

Gouvernement du Canada | Natural Sciences and Engineering Research Council of Canada

Gouvernement du Canada | Canadian Institutes of Health Research

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.02665-16

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