ACK Family Tyrosine Kinase Activity Is a Component of Dcdc42 Signaling during Dorsal Closure in
Drosophila melanogaster
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Published:2002-06
Issue:11
Volume:22
Page:3685-3697
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ISSN:0270-7306
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Container-title:Molecular and Cellular Biology
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language:en
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Short-container-title:Mol Cell Biol
Author:
Sem Kai Ping1, Zahedi Baharak1, Tan Ivan2, Deak Maria3, Lim Louis24, Harden Nicholas1
Affiliation:
1. Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, British Columbia V5A 1S6, Canada 2. Glaxo-IMCB Laboratory, Institute of Molecular and Cell Biology, Singapore 117609, Republic of Singapore 3. MRC Protein Phosphorylation Unit, Department of Biochemistry, University of Dundee, Dundee DD1 5EH 4. Department of Neurochemistry, Institute of Neurology, London WC1 1PJ, United Kingdom
Abstract
ABSTRACT
We have characterized
Drosophila melanogaster
ACK (DACK), one of two members of the ACK family of nonreceptor tyrosine kinases in
Drosophila
. The ACKs are likely effectors for the small GTPase Cdc42, but signaling by these proteins remains poorly defined. ACK family tyrosine kinase activity functions downstream of
Drosophila
Cdc42 during dorsal closure of the embryo, as overexpression of DACK can rescue the dorsal closure defects caused by dominant-negative Dcdc42. Similar to known participants in dorsal closure, DACK is enriched in the leading edge cells of the advancing epidermis, but it does not signal through activation of the Jun amino-terminal kinase cascade operating in these cells. Transcription of
DACK
is responsive to changes in Dcdc42 signaling specifically at the leading edge and in the amnioserosa, two tissues involved in dorsal closure. Unlike other members of the ACK family, DACK does not contain a conserved Cdc42-binding motif, and transcriptional regulation may be one route by which Dcdc42 can affect DACK function. Expression of wild-type and kinase-dead
DACK
transgenes in embryos, and in the developing wing and eye, reveals that ACK family tyrosine kinase activity is involved in a range of developmental events similar to that of Dcdc42.
Publisher
American Society for Microbiology
Subject
Cell Biology,Molecular Biology
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