Affiliation:
1. Department of Molecular and Cell Biology, University of Texas at Dallas, Dallas, Texas 75080
Abstract
ABSTRACT
YqjH and YqjW are
Bacillus
subtilis
homologs of the UmuC/DinB or Y superfamily of DNA polymerases that are involved in SOS-induced mutagenesis in
Escherichia
coli
. While the functions of YqjH and YqjW in
B
.
subtilis
are still unclear, the comparisons of protein structures demonstrate that YqjH has 36% identity to
E
.
coli
DNA polymerase IV (DinB protein), and YqjW has 26% identity to
E
.
coli
DNA polymerase V (UmuC protein). In this report, we demonstrate that both YqjH and the products of the
yqjW
operon are involved in UV-induced mutagenesis in this bacterium. Furthermore, resistance to UV-induced damage is significantly reduced in cells lacking a functional YqjH protein. Analysis of stationary-phase mutagenesis indicates that absences of YqjH, but not that of YqjW, decreases the ability of
B
.
subtilis
to generate revertants at the
hisC952
allele via this system. These data suggest a role for YqjH in the generation of at least some types of stationary-phase-induced mutagenesis.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
90 articles.
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