Flow Cytometry Analysis of HIV-1 Env Conformations at the Surface of Infected Cells and Virions: Role of Nef, CD4, and SERINC5-Reference-Cited by-同舟云学术

Flow Cytometry Analysis of HIV-1 Env Conformations at the Surface of Infected Cells and Virions: Role of Nef, CD4, and SERINC5

Published:2020-02-28 Issue:6 Volume:94 Page:
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Staropoli Isabelle¹,Dufloo Jérémy¹²³,Ducher Anaïs¹,Commere Pierre-Henri⁴,Sartori-Rupp Anna⁵,Novault Sophie⁴,Bruel Timothée¹³,Lorin Valérie⁶,Mouquet Hugo⁶,Schwartz Olivier¹^ORCID,Casartelli Nicoletta¹

Affiliation:

1. Institut Pasteur, Virus and Immunity Unit, CNRS-UMR3569, Paris, France

2. Paris Diderot University, Sorbonne Paris Cité, Paris, France

3. Vaccine Research Institute, Créteil, France

4. Institut Pasteur, Flow Cytometry Platform, Paris, France

5. Institut Pasteur, Unit of Technology and Service Ultra-Structural Bio-Imaging, Paris, France

6. Institut Pasteur, Laboratory of Humoral Immunology, Inserm U1222, Paris, France

Abstract

HIV-1 Env conformation is one of the key parameters determining viral infectivity. The flow virometry-based assay developed in this study allows for the characterization of proteins incorporated in HIV-1 particles. We studied the conformation of HIV-1 Env and the impact that the viral protein Nef and the cellular proteins CD4 and SERINC5 have on Env accessibility to antibodies. Our assay permitted us to highlight some noticeable differences in the conformation of Env between producer cells and viral particles. It contributes to a better understanding of the actual composition of HIV-1 particles.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JVI.01783-19

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