Affiliation:
1. Biological Adaptation Branch, Ames Research Center, National Aeronatics and Space Administration, Moffett Field, California 94035
2. Department of Biology, San Jose State College, San Jose, California 95114
Abstract
A lytic bacteriophage isolated from sewage was found to attack strains of
Aerobacter aerogenes, Escherichia coli
, and
Klebsiella pneumoniae
, but not members of the genera
Salmonella, Proteus
, and
Serratia.
The phage, designated φmp, contained deoxyribonucleic acid with a 50% guanine plus cytosine ratio and a molecular weight of 23.1 × 10
6
daltons. Single-step growth experiments of φmp plated at 37 C on
A. aerogenes
A2 gave a mean latent period of 20 min, an average burst size of 103 plaque-forming units/infected cell, and an average adsorption rate constant of 3 × 10
−10
ml/min. Electron microscopy of φmp revealed a phage with a flexible tail (165 nm long and 6 nm wide). The phage head had a hexagonal outline (62 nm in diameter).
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
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