Safranal'ın Servikal Kanseri C-4 I Hücre Hattı Üzerindeki Antiproliferatif Etkisinin Değerlendirilmesi
Author:
ATHOUMANI ALI Souandaou1ORCID, ÖZCAN Fatma Gül2ORCID, KARABULUT-BULAN Omur2ORCID
Affiliation:
1. İstanbul üniversitesi 2. İSTANBUL ÜNİVERSİTESİ, FEN FAKÜLTESİ
Abstract
Aim: Safranal is a bioactive compound responsible for the aroma propriety of Crocus sativus. Many studies have shown the antioxidant activity of safranal besides some pharmacological properties, including its anti-inflammatory effect. Our study aimed on identifying the cytotoxic effects of safranal on the C-4 I cell line of cervical cancer.
Methods: The cytotoxic effect of safranal on the C-4 I cell line was determined after incubating the cells for certain durations (2 to 72 hours) and concentrations (25 to 800 µM). After incubation, cell viability and anti-proliferation effect of safranal were determined respectively by Mitochondrial Dehydrogenase and Lactate dehydrogenase enzymes activities assays. Additionally, morphological changes occurring during incubation in cells were examined with inverted and optical microscope using Giemsa staining.
Results: According to the results, compared to Control group, the % viability of treated cells was decreased depending on concentration and the incubation time, and safranal significantly inhibited the growth of C-4 I cells (p
Publisher
Giresun Üniversitesi
Reference33 articles.
1. Bakshi, H. A., Quinn, G. A., Nasef, M. M., Mishra, V., Aljabali, A. A. A., El-Tanani, M., Serrano-Aroca, Á., Webba Da Silva, M., McCarron, P. A., & Tambuwala, M. M. (2022). Crocin Inhibits Angiogenesis and Metastasis in Colon Cancer via TNF-α/NF-kB/VEGF Pathways. Cells, 11(9), 1502. https://doi.org/10.3390/cells11091502. 2. Burak, A. K. Ç. A., & Güven, L. (2023). Kanser Tedavisinde Kullanılan Tıbbi Bitkiler. Sağlık Bilimlerinde Öncü Ve Çağdaş Çalışmalar, 641-679. https://scholar.google.com/citations?view_op=view_citation&hl=tr&user=pHHgdH8AAAAJ&citation_for_view=pHHgdH8AAAAJ:MXK_kJrjxJIC 3. Cerdá-Bernad, D., Valero-Cases, E., Pastor, J. J., & Frutos, M. J. (2022). Saffron bioactives crocin, crocetin and safranal: effect on oxidative stress and mechanisms of action. Critical reviews in food science and nutrition, 62(12), 3232–3249. https://doi.org/10.1080/10408398.2020.1864279. 4. Chaiboonchoe, A., Khraiwesh, B., Murali, C., Baig, B., Tarazi, H., Alzahmi, A., Nelson, D. R., Greish, Y. E., Ramadan, W., & Amin, A. (2018). Safranal induces DNA double-strand breakage and ER-stress-mediated cell death in hepatocellular carcinoma cells. Scientific Reports, 8(1), 1-15. https://doi.org/10.1038/s41598-018-34855-0 5. Cheriyamundath, S., Choudhary, S., & Lopus, M. (2017). Safranal Inhibits HeLa Cell Viability by Perturbing the Reassembly Potential of Microtubules. Phytotherapy Research, 32(1), 170-173. https://doi.org/10.1002/ptr.5938
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