Comparative Molecular Genetic Characteristics of Mycobacterium tuberculosis Cultures Isolated in the European Part of the Russian Federation in 1998–2003 and 2016

Comparative Molecular Genetic Characteristics of Mycobacterium tuberculosis Cultures Isolated in the European Part of the Russian Federation in 1998–2003 and 2016–2021

Published:2023-07-04 Issue:3 Volume:101 Page:27-36
ISSN:2542-1506
Container-title:Tuberculosis and Lung Diseases
language:
Short-container-title:Tuberk. bolezni lëgk.

Author:

Andreevskaya S. N.¹,Larionova E. E.¹,Kiseleva E. A.¹,Chernousova L. N.¹,Ergeshov A. Е.²

Affiliation:

1. Central Tuberculosis Research Institute

2. Central Tuberculosis Research Institute; A. I. Yevdokimov Moscow State University of Medicine and Dentistry

Abstract

The objective: to evaluate changes in detection frequency of Mycobacterium tuberculosis of the main phylogenetic lines with various genetic determinants of resistance to rifampicin, isoniazid and fluoroquinolones in 1998–2003 and 2016–2021.Subjects and Methods. 965 mycobacterial cultures were studied, those cultures were isolated from pulmonary tuberculosis patients in 1998–2003 and 2016–2021. The spoligotypes of isolated Mycobacterium tuberculosis cultures and presence of gene mutations associated with resistance to isoniazid, rifampicin, and fluoroquinolones were determined.Results. In 2016–2021 versus 1998–2003, the incidence of Beijing subline increased from 50,72% to 64,60%. Within Euro-American line, the detection frequency of subline LAM9 decreased (from 40,00% to 14,81%), while the detection frequency of sublineT1 increased (from 27,11% to 41,36%). Mycobacterium tuberculosis with MDR genotype rpoB531_Ser->Leu + katG315_Ser-> Thr [1] was selected. Mycobacterium tuberculosis with genotypic resistance to fluoroquinolones was detected only in cultures of 2016–2021, and Mycobacterium tuberculosis with preXDR genotype was significantly more common in Beijing subline compared to Euro-American line (21,67% vs. 3,73%, p <0,05).

Publisher

LLC "Medical Knowledge and Technologies"

Subject

General Medicine

Reference22 articles.

1. Andreevskaya S. N., Chernousova L. N., Zemskova Z. S., Larionova E. E., Smirnova T. G. Biological properties of strains M. tuberculosis cluster W. Problemy Tuberkuleza i Bolezney Legkikh, 2008, vol. 85, no. 10, pp. 45–50. (In Russ.)

2. Andreevskaya S. N., Chernousova L. N., Smirnova T. G., Larionova E. E., Kuzmin A. V. Transmission of tuberculous mycobacteria strains due to migration in the Russian Federation (using the example of migration from Caucasus to Moscow and Moscow Region). Problemy Tuberkuleza i Bolezney Legkikh, 2006, no. 1, pp. 29–35. (In Russ.)

3. Vasilyeva I. A., Testov V. V., Sterlikov S. A. Tuberculosis situation in the years of the COVID-19 pandemic – 2020–2021. Tuberculosis and Lung Diseases, 2022, vol. 100, no. 3, pp. 6–12. (In Russ.)

4. Tuberkulez v Rossiyskoy Federatsii 2011 g. Аnaliticheskiy obzor statisticheskikh pokazateley, ispolzuemykh v Rossiyskoy Federatsii i v mire. [Tuberculosis in the Russian Federation in 2011. Analytic review of statistic rates used in the Russian Federation and in the world]. Moscow, 2013, 280 p.

5. Chernousova L. N., Golyshevskaya V. I., Erokhin V. V., Kuznetsov S. I., Zakharova S. M., Melentiev A. S., Fedorin I. M., Nikolaevsky V. V., Ruddy M., Balabanova Ya. M., Drobnevsky F. Prevalence of Mycobacterium tuberculosis strains of Beijing family and risk factors of their transmission in Samara Region. Problemy Tuberkuleza i Bolezney Legkikh, 2006, no. 2, рp. 31–37. (In Russ.)

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