Author:
Juengel Jennifer L,French Michelle C,Quirke Laurel D,Kauff Alexia,Smith George W,Johnstone Peter D
Abstract
We hypothesised that cocaine- and amphetamine-regulated transcript (CARTPT) would be differentially expressed in ewes with differing ovulation rates. Expression of mRNA forCARTPT, as well asLHCGR,FSHR,CYP19A1andCYP17A1was determined in antral follicles ≥1 mm in diameter collected during the follicular phase in ewes heterozygous for the Booroola and Inverdale genes (I+B+; average ovulation rate 4) and ++ contemporaries (++; average ovulation rate 1.8). In ++ ewes (n = 6),CARTPTwas expressed in small follicles (1 to <3 mm diameter), where 18.8 ± 2.5% follicles expressedCARTPT. CART peptide was also detected in follicular fluid of some follicles of ++ ewes. In I+B+ ewes, 5/6 ewes did not have any follicles that expressedCARTPT, and no CART peptide was detected in any follicle examined. Expression pattern ofCYP19A1differed between I+B+ and ++ ewes with an increased percentage of small and medium follicles (3 to <4.5 mm diameter) but decreased percentage of large follicles (≥4.5 mm diameter) expressingCYP19A1in the I+B+ ewes. Many of the large follicles from the I+B+ ewes appeared non-functional and expression ofLHCGR,FSHR,CYP17A1andCYP19A1was less than that observed in ++ ewes. Expression ofFSHRandCYP17A1was not different between groups in small and medium follicles, butLHCGRexpression was approximately double in I+B+ ewes compared to that in ++ ewes. Thus, ewes with high ovulation rates had a distinct pattern of expression ofCARTPTmRNA and protein compared to ewes with normal ovulation rates, providing evidence for CART being important in the regulation of ovulation rate.
Subject
Cell Biology,Obstetrics and Gynaecology,Endocrinology,Embryology,Reproductive Medicine
Cited by
11 articles.
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