A high-precision automated liquid pipetting device with an interchangeable tip

Abstract

Liquid handling is a necessary act to deal with liquid samples from scientific labs to industry. However, existing pipetting devices suffer from inaccuracy and low precision when dealing with submicroliter liquids, which significantly affect their applications in low-volume quantitation. In this article, we present an automated liquid pipetting device that can aspirate liquid from microplates and dispense nanoliter droplets with high precision. Liquid aspiration is realized by using a micropump and a solenoid valve, and on-demand nanoliter droplet printing is realized by using a low-cost and interchangeable pipette tip combined with a piezoelectric actuator. Based on the microfluidic printing technology, the volumetric coefficient of variation of the dispensed liquid is less than 2% below 1 µl. A demonstration of concentration dilution for quantitative analysis has been successfully performed using the automated liquid pipetting device, demonstrating its potential in low-volume liquid handling for a wide range of biomedical applications.