A KINETIC ANALYSIS OF THE ENDOGENOUS RESPIRATION OF BAKERS' YEAST

Author:

Stier T. J. B.1,Stannard J. N.1

Affiliation:

1. From the Biological Laboratories, Harvard University, Cambridge

Abstract

The process of endogenous respiration of two strains of bakers' yeast, Saccharomyces cerevisiae, was examined kinetically. The rate of respiration with respect to time in a non-nutrient medium was found to exhibit two phases: (a) a period of constant rate of O2 consumption and CO2 production (R.Q. = 1) characteristic of cells with ample concentrations of stored material; (b) a first order decline in rate of respiration with respect to time, where the rate was proportional to the concentration of some substrate, S. (R.Q. = 1 throughout second phase.) The nature of this substrate was reexamined and the evidence summarized confirms the notion that it is a carbohydrate, probably glycogen. These phases of endogenous respiration were shown to depend upon the age of the culture and the amount of substrate available.

Publisher

Rockefeller University Press

Subject

Physiology

Cited by 45 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. ENDOGENOUS METABOLISM OF RESERVE DEPOSITS IN YEAST*;Annals of the New York Academy of Sciences;2006-12-15

2. The Mechanism of Fertilization in Metazoa;Advances in Enzymology - and Related Areas of Molecular Biology;2006-11-22

3. Membrane potentials in respiring and respiration-deficient yeasts monitored by a fluorescent dye;Biochimica et Biophysica Acta (BBA) - Bioenergetics;1981-09

4. The influence of conditions of growth on the endogenous metabolism ofSaccharomyces cerevisiae: Effect on respiratory activity;Antonie van Leeuwenhoek;1977-09

5. Chapter IX Cellular Electrophysiology;Methods in Microbiology;1972

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