Antioxidant and Antidiabetic Activities of Biologically Synthesized Silver Nanoparticles using Linumusitatissimum Extract

Abstract

This research work was mainly focused to study the anti-oxidant and anti-diabetic activities of biologically synthesis of silver nanoparticles (AgNPs) from the flaxseed extract of Linumusitassimum. Qualitative tests identify the presence of phytochemicals in the flaxseed extract and its results showed the presence of tannins, terpenoids, saponins, flavonoids, steroids, cardiac glycosides, anthraquinones, coumarins, xanthoproteins, alkaloids, emodins, and carbohydrate in it. Preliminarily AgNPs formation is confirmed by the UV spectra and it showed maximum adsorption band at 438nm. FT-IR spectroscopic studies reveal the Phyto-constituents which are involved in the reduction of silver (Ag+1) into silver nanoparticles (Ag0). The spherical shapes of AgNPs are observed with crystalline nature are found in the aid of SEM and XRD analysis. Synthesized AgNPs have the maximum percentage of a silver element which is examined by the EDX analysis. The in-vitro antioxidant and antidiabetic activities of L. usitatissimummediated AgNPs were analyzed by using the DPPH, alpha-amylase, and alpha glycosides assays respectively. The DPPH result shows that the AgNPs possess 59.01% of radical scavenging property and the standard ascorbic acid reveals 48.63% at 100µg/ml concentration. Similarly in anti-diabetic activity, AgNPs shows the maximum inhibition of 79.84% in the alpha-amylase assay, and for alpha-glucosidase, AgNPs showed 58.86% at 100µg/ml concentration.