Preliminary Screening for In-Vitro Antioxidant and Anticancer Potentials in Whole Plant Fractions of Cayratia Auriculata (Vitaceae)

Abstract

Failure of natural homeostasis of healthy cell due to hyperproliferative nature of cancer, resulting in stimulation of various genes which are intensively participated in cell cycle, survival, angiogenesis and metastasis. Our study focused on the in-vitro antioxidant and cytotoxic potentials of the whole plant fractions of Cayratia auriculata, a medicinal plant belongs to Vitaceae family. The whole plant material was shade dried and powdered, fractions were prepared by using soxhlet extraction technique with the ascending order of polarity such as hexane < chloroform, < ethyl acetate < methanol. Screening for phytoconstituents in fractions was carried with standard biochemical instigations. Quantitative investigation was done by using different assays such as total phenolic content (TFC), ferric reducing antioxidant power (FRAP) assay, nitric oxide scavenging activity, 2,2 -Diphenyl-1-picrylhydrazyl (DPPH) assay and total antioxidant activity (TAC) to reveal antioxidant capacity. In-vitro cytotoxicity activity on A549 lung cancer cell line was evaluated by (3-(4, 5-dimethyl thiazole-2yl)-2, 5-diphenyl tetrazolium bromide) MTT assay. Phytochemical analysis of all four fractions showed the existence of varying degree of secondary bioactive metabolites but methanol fraction exhibited richness in phytoconstituents. Methanol fraction revealed good total phenolic content, potent antioxidant potential in FRAP, DPPH, Nitric oxide scavenging activity and total antioxidant activity in contrast to other tested fractions. MTT assay revealed that methanol fraction C. auriculata has strongest cytotoxic effect towards (A549) lung cancer cell line with an inhibitory concentration (IC50) value of 115.14 µg/ml. The results of present study indicate that different fractions of C. auriculata showed the existence of varying degree of phytochemicals, total phenolic content and dose dependent antioxidant activity. Methanolic fraction revealed richness in phytochemicals, total phenolic content, potent antioxidant, and anticancer property (in- vitro).