Phase-dependent expression profiling and quantification of several growth factors in liver regeneration after partial hepatectomy
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Published:2021-01-01
Issue:2
Volume:3
Page:
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ISSN:2709-2798
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Container-title:Pakistan BioMedical Journal
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language:
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Short-container-title:PBMJ
Author:
Hussain Adeela,Ali Gibran,Afzal Muhammad Awais,Tayyeb Asima,Tariq Muhammad Akram,Akram Shehla J,Ahmad Fridoon Jawad,Akram Javed
Abstract
Growth factors are the potential operational members which control different phases of liverregeneration. Different growth factors have expression regulation in the whole process relating todifferent phases of liver regeneration. Objective: To assess the expression regulation of differentgrowth factors and cytokines involved in liver regeneration in a phase-dependent manner. Methods:Blood and liver samples were collected and analyzed on 1st, 3rd, 5th, 7th and 14th postoperative days after50% Partia hepatectomy (PHx). Results: Steady increase of liver regeneration rate was recorded from90.8% (1st day) to 97.9% (7th day). Liver function tests further confirmed the steady liver recovery in PHxmice. Several growth factors such as HGF and VEGF exhibited an up-regulation till 5th day and latergradual decrease till 14th day compared to control mice. Albumin, CK18 and CK19 showed sequentialexpression increase from 1st to 14th day compared to AFP and HNF-4α upregulated until 5th and 1st day,respectively. Quantification of these growth factors further confirm our results. Conclusions:Conclusively, these results highlight a phase-dependent regulation and role of growth factors in liverregeneration and recovery .
Publisher
CrossLinks International Publishers
Subject
General Engineering
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