The effect of retinol and retinoic acid on physiological and biochemical changes in retinol-deficient rats

Abstract

1. The effects of retinol and retinoic acid supplementation of retinol-deficient rats were studied for a variety of metabolic processes shown to be affected by retinol deficiency.2. Retinol-deficient rats were found to have decreased body-weight, liver and testes weights, a degeneration of testicular germinal cells, an increased incorporation of labelled choline into liver and testes phospholipids, an increased protein synthetic activity (in vitro) of liver ribosomes, an increased transfer-RNA methyltransferase activity in liver and a decreased activity in testes, an increased DNA content of testicular nuclei, and a decreased uptake of [3H]thymidine by testicular nuclear DNA.3. In retinol-deficient rats supplemented for 8 weeks with retinol these changes were reversed, measurements returning to control levels.4. In retinol-deficient rats supplemented for 8 weeks with retinoic acid all changes were reversed except those in the testes.5. Testicular signs of retinol deficiency appeared to be delayed when retinoic acid was added to the retinol-deficient diet of weanling rats. This suggests a sparing action of retinoic acid on the rat's utilization of retinol.6. Suggestions are offered as to why retinoic acid will support growth and development but not spermatogenesis in the rat.