Effects of reducing the starch content in oat-based diets with cellulose on jejunal flow and absorption of glucose over an isolated loop of jejunum in pigs

Abstract

Four pigs fitted with two sets of re-entrant cannulas in the upper jejunum were used to study the effect of two oat-flour (F)- and oat-bran (B)-based diets without or with (FC and BC respectively) addition of 148 g wood cellulose/kg on jejunal flow and absorption of glucose over an isolated loop of jejunum. Mean transit time (MTT) of flow from the proximal re-entrant cannula increased from approximately 1·5 h with diet F to 2 h when feeding diet BC. Both the replacement of oat flour by oat bran and the supplementation of the diets with cellulose led to a significantly longer MTT for dry matter and the carbohydrates, except that cellulose did not have a significant effect on the MTT for the total non-starch polysaccharides. Only the addition of cellulose significantly delayed gastric emptying of the added liquid-phase (Polyethylene glycol 4000) and solid-phase (Cr2O3) markers, whereas no effect of the oat source used was seen. Feeding diets with a higher level of dietary fibre (DF) and lower content of starch, obtained by substitution with cellulose or by feeding oat bran instead of oat flour, reduced the recovery of starch from an isolated loop of jejunum. Consequently, the quantitative absorption of starch was not significantly different between diets when starch was related to the recovery of external markers. However, when related to the recovery of arabinoxylan (AX) there was a significantly lower absorption of starch from the bran-based diets in comparison with the flour-based diets. The capacity to digest and absorb the large quantities of starch entering the duodenum after a carbohydrate-rich meal appears to be the limiting factor for absorption in the upper jejunum. Therefore, any effect of DF on the rate of absorption of glucose is presumably an effect on gastric emptying rather than displacement of the site of starch absorption in the small intestine.