Estimation of tissue protein synthesis in sheep during sustained elevation of plasma leucine concentration by intravenous infusion

Abstract

1. The fractional rate of protein synthesis (FSR) was determined in skeletal muscle, liver, rumen and cardiac muscle of wether sheep by continuous intravenous infusion of L–[4,5–3H]leucine accompanied by infusion of 0, 7.6, 15.2 or 22.8 mmol leucine/h (three sheep per treatment). FSR was calculated assuming plasma (ksp) or intracellular (ksi) leucine-specific radioactivity (SRA) was representative of the leucine precursor pool SRA for protein synthesis.2. Plasma leucine concentration (plateau) was linearly related to leucine infusion rate, 22.8 mmol/h evoking tenfold increase in plasma concentration.3. Difference between plasma leucine SRA and intracellular leucine SRA in all tissues diminished as plasma leucine concentration increased.4. There were significant differences between ksiand kapestimates for liver and rumen in control sheep.5. As leucine infusion rate increased, differences between kriand kag, diminished in all tissues. With increasing leucine infusion, in liver kstdecreased and kspwas increased, in rumen kgedecreased and kspwas stable, while in cardiac and skeletal muscle ksiand kspboth increased.6. At a leucine infusion rate of 22.8 mmol/h, mean kap, and kstrespectively were: rumen 1 1 (SE 2), 13 (SE 1); liver 19 (SE 2), 21 (SE 2); cardiac muscle 3–6 (SE 0.4), 3.8 (SE 0.3); skeletal muscle 4.1 (SE 0.2), 4.5 (SE 0.5) and did not differ significantly in any tissue.