ELISA of Picogram Quantities of Aflatoxin M1 in Urine and Milk

Author:

HU WEN J.1,WOYCHIK NANCY1,CHU FUN S.1

Affiliation:

1. Department of Food Microbiology and Toxicology, University of Wisconsin, Madison, Wisconsin 53706

Abstract

Pretreatment of milk or urine with a Sep-Pak C-18 reversed-phase cartridge permits detection by direct enzyme-linked immunosorbent assay (ELISA) of 10 to 25 pg/ml of aflatoxin M1 added to samples. The samples were first loaded to the Sep-Pak cartridge followed by washing with 10% acetonitrile in water. Aflatoxin M1 was eluted from the cartridge with 30% acetonitrile in water and then transferred to the methylene chloride. After the solvent was removed, the sample was redissolved in 50 μl of methanol, diluted with 0.45 ml of buffer, and finally subjected to the ELISA. The recovery of aflatoxin M1 added to milk and urine in the range of 10 to 250 pg/ml was between 77 to 110%.

Publisher

International Association for Food Protection

Subject

Microbiology,Food Science

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