Rapid Detection and Identification of Lactobacillus spp. in Dairy Products by Using the Polymerase Chain Reaction-Reference-Cited by-同舟云学术

Rapid Detection and Identification of Lactobacillus spp. in Dairy Products by Using the Polymerase Chain Reaction

Published:1996-10-01 Issue:10 Volume:59 Page:1031-1036
ISSN:0362-028X
Container-title:Journal of Food Protection
language:en
Short-container-title:

Author:

DRAKE MARYANNE¹,SMALL CHRISTOPHER L.²,SPENCE KEMET D.²,SWANSON BARRY G.¹

Affiliation:

1. 1Department of Food Science and Human Nutrition, Washington State University, Pullman, Washington 99164-6376, USA

2. 2Department of Microbiology, Washington State University, Pullman, Washington 99164-6376, USA

Abstract

Species-specific primers for use in the polymerase chain reaction (PCR) were designed to differentially amplify DNA from the common dairy lactobacillus species Lactobacillus casei, Lactobacillus delbrueckii, Lactobacillus helveticus, and Lactobacillus acidophilus. A method for rapid extraction of bacterial DNA from dairy products was developed. The sensitivity of bacterial DNA extraction from food and subsequent amplification by PCR was 100 cells total. Lactobacillus DNA was extracted and identified from commercial yoghurts, acidophilus milk, and cheeses. The methodology allows the presumptive identification of dairy lactobacilli in less than 6 hours.

Publisher

International Association for Food Protection

Subject

Microbiology,Food Science

Link

http://meridian.allenpress.com/jfp/article-pdf/59/10/1031/1659949/0362-028x-59_10_1031.pdf

Cited by 84 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Quantification of Lactobacillus delbrueckii subsp. Bulgaricus and its applicability as a tracer for studying contamination spread on environmental surfaces;Building and Environment;2021-06

2. Gut bacterial profile in Indian children of varying nutritional status: a comparative pilot study;European Journal of Nutrition;2021-04-30

3. Microbiota of Sardinian Goat’s Milk and Preliminary Characterization of Prevalent LAB Species for Starter or Adjunct Cultures Development;BioMed Research International;2019-07-08

4. Improvement of Ayran quality by the selection of autochthonous microbial cultures;Food Microbiology;2016-12

5. The use of PCR-DGGE to determine bacterial fingerprints for poultry and red meat abattoir effluent;Letters in Applied Microbiology;2015-11-25