Occurrence of Fusarium Mycotoxins in Rice and Its Milling By-Products in Korea

Abstract

A total of 201 samples of brown rice, polished rice, and two types of by-products, blue-tinged rice and discolored rice, were collected from rice stores maintained at 51 rice processing complexes in Korea. These samples were analyzed for the presence of Fusarium mycotoxins such as deoxynivalenol (DON), nivalenol (NIV), and zearalenone (ZEA). Contaminants (and their ranges) found in discolored rice samples were DON (59 to 1,355 ng g−1), NIV (66 to 4,180 ng g−1), and ZEA (25 to 3,305 ng g−1); those found in blue-tinged (less-ripe) rice were DON (86 to 630 ng g−1), NIV (50 to 3,607 ng g−1), and ZEA (26 to 3,156 ng g−1). Brown rice samples were contaminated mostly with NIV and ZEA (52 to 569 ng g−1 and 47 to 235 ng g−1, respectively). Polished rice samples were largely free from mycotoxins, although one sample was contaminated with NIV (77 ng g−1). When the fungal flora associated with each rice sample was investigated, blue-tinged rice was the most often contaminated with Fusarium graminearum (3.8%), followed by the discolored rice (2.4%) and brown rice (1.6%) samples. Using PCR, toxin genotyping of 266 isolates of F. graminearum revealed that most isolates (96%) were NIV producers. In conclusion, this survey is the first report of the cocontamination of Korean rice and its by-products with trichothecenes and ZEA. Importantly, it also provides new information on the natural contamination of rice by Fusarium mycotoxins.