Combined Effects of Carbonation with Heating and Fatty Acid Esters on Inactivation and Growth Inhibition of Various Bacillus Spores

Author:

KLANGPETCH WANNAPORN1,NAKAI TOMOE1,NOMA SEIJI2,IGURA NORIYUKI2,SHIMODA MITSUYA2

Affiliation:

1. 1Graduate School of Bioresources and Bioenvironmental Sciences, Kyushu University, Fukuoka 812-8581, Japan

2. 2Division of Bioresources and Bioenvironmental Sciences, Kyushu University, Fukuoka 812-8581, Japan

Abstract

The effects of carbonation treatment (1 to 5 MPa, 30 min) plus heat treatment (30 to 80°C, 30 min) in the presence of various fatty acid esters (FAEs; 0.05 and 0.1%, wt/vol) on counts of viable Bacillus subtilis spores were investigated. FAEs or carbonation alone had no inactivation or growth inhibition effects on B. subtilis spores. However, carbonation plus heat (CH; 80°C, 5 MPa, 30 min) in the presence of mono- and diglycerol fatty acid esters markedly decreased counts of viable spores, and the spore counts did not change during storage for 30 days. The greatest decrease in viable spore counts occurred in the presence of monoglycerol fatty acid esters. Under CH conditions, inactivation and/or growth inhibition occurred at only 80°C and increased with increasing pressure. The greatest decrease in spore counts (more than 4 log units) occurred with CH (80°C, 5 MPa, 30 min) in the presence of monoglycerol fatty acid esters. However, this treatment was less effective against Bacillus coagulans and Geobacillus stearothermophilus spores.

Publisher

International Association for Food Protection

Subject

Microbiology,Food Science

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