Microtiter Technique for Enumeration of Mesophiles, Psychrotrophs, and Coliforms in Raw and Pasteurized Milk

Abstract

The Microtiter Count Method was compared with the Standard Plate Count (SPC) method in evaluating mesophile, psychrotroph, and coliform counts for raw and pasteurized milk samples. Statistical analysis showed that the Microtiter Count Method was reliable when compared with the SPC for making viable cell counts on these products. The Microtiter Count Method is advantageous because it saves time, space, and material; this method should be useful for developing countries where availability of testing materials, manpower, and costs are limiting factors in surveillance of microbial quality.