Detection of Salmonellae and Other Enterobacteriaceae by Commercial Deoxyribonucleic Acid Hybridization and Enzyme Immunoassay Kits

Abstract

Cultures of 294 Salmonella and 100 other Enterobacteriaceae isolates were comparatively analyzed by the GENE-TRAKR Salmonella Assay, the ReportR Salmonella Visual Immunoassay, and the Organon Teknika Salmonella-TekR assay. Of the Salmonella isolates, 99.0% were reactive in the GENE-TRAK assay, 98.0% were reactive in the Report assay and 100% were reactive in the Organon Teknika assay. The three strains missed by the GENE-TRAK method belonged to Salmonella subgenus V. Four of seven strains that were nonreactive by the Report assay were nonmotile isolates; some nonmotile isolates were reactive. Of the non-Salmonella isolates assayed, 6.0% were consistently positive by the Report Method and 16.0% were consistently positive by the Salmonella-Tek method, while none were consistently positive by the GENE-TRAK method. Variable assay results were obtained with 19.0% of the strains using the Report assay, 20.0% of the strains in the Salmonella-Tek assay, and 7.0% of the strains by the GENE-TRAK assay.