Isolation of a Virulent Lactobacillus brevis Phage and Its Application in the Control of Beer Spoilage

Author:

DEASY THERESE1,MAHONY JENNIFER1,NEVE HORST2,HELLER KNUT J.2,VAN SINDEREN DOUWE13

Affiliation:

1. 1Department of Microbiology, Kiel, Germany

2. 2Max Rubner Institut, Kiel, Germany

3. 3Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland

Abstract

Beer quality can be compromised by the growth of certain lactobacilli, in particular Lactobacillus brevis and Lactobacillus plantarum, and various strategies have been used to control such bacterial spoilage. Biocontrol by means of bacteriophage is a reemerging approach for the suppression of spoilage bacteria in food and beverage matrices. A virulent phage capable of infecting L. brevis beer-spoilage strains was isolated and morphologically assessed by electron microscopy. The myophage SA-C12 was shown to be stable in beer and capable of controlling the growth of its host, L. brevis strain 56, in commercial beer. The results of this study indicate that bacteriophage-based treatments may be used as an alternative and natural strategy for the control of bacterial contamination of beer.

Publisher

International Association for Food Protection

Subject

Microbiology,Food Science

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