Survival of Shiga Toxin–Producing Escherichia coli in Various Wild Animal Feces That May Contaminate Produce

Author:

TOPALCENGIZ ZEYNAL12,JEAMSRIPONG SAHARUETAI345,SPANNINGER PATRICK M.6,PERSAD ANIL K.78,WANG FEI9,BUCHANAN ROBERT L.910,LeJEUNE JEFF8,KNIEL KALMIA E.6,JAY-RUSSELL MICHELE T.311,DANYLUK MICHELLE D.212

Affiliation:

1. Department of Food Engineering, Faculty of Engineering and Architecture, Muş Alparslan University, Muş 49250, Turkey (ORCID: https://orcid.org/0000-0002-2113-7319 [Z.T.])

2. Department of Food Science and Human Nutrition, Citrus Research and Education Center, University of Florida, Lake Alfred, Florida 33850, USA

3. Western Institute for Food Safety and Security, University of California Davis, Davis, California 95618, USA

4. ORCID: https://orcid.org/0000-0001-7332-1647 [S.J.]

5. Research Unit in Microbial Food Safety and Antimicrobial Resistance, Department of Veterinary Public Health, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand

6. Department of Animal and Food Sciences, University of Delaware, Newark, Delaware 19716, USA

7. School of Veterinary Medicine, Faculty of Medical Sciences, The University of the West Indies, St. Augustine, Eric Williams Medical Sciences Complex, Mount Hope, Trinidad and Tobago (ORCID: https://orcid.org/0000-0002-1306-325X [A.K.P.])

8. Food Animal Health Research Program, The Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, Ohio 44691, USA

9. Department of Nutrition and Food Science and Center for Food Safety and Security Systems, University of Maryland, College Park, Maryland 20742, USA

10. (ORCID: https://orcid.org/0000-0002-7604-4048 [R.L.B.])

11. ORCID: https://orcid.org/0000-0001-9849-8086 [M.T.J.R.]

12. (ORCID: https://orcid.org/0000-0001-5780-7911 [M.D.D.])

Abstract

ABSTRACT Domestic and wild animal intrusions are identified as a food safety risk during fresh produce production. The purpose of this study was to evaluate the survival of Shiga toxin–producing Escherichia coli (STEC) in cattle, feral pig, waterfowl, deer, and raccoon feces from sources in California, Delaware, Florida, and Ohio. Fecal samples were inoculated with a cocktail of rifampin-resistant STEC serotypes (O103, O104, O111, O145, and O157) (104 to 106 CFU/g of feces). Inoculated feces were held at ambient temperature. Populations of surviving cells were monitored throughout 1 year (364 days), with viable populations being enumerated by spread plating and enrichment when the bacteria were no longer detected by plating. Representative colonies were collected at various time intervals based on availability from different locations to determine the persistence of surviving STEC serotypes. Over the 364-day storage period, similar survival trends were observed for each type of animal feces from all states except for cattle and deer feces from Ohio. STEC populations remained the highest in cattle and deer feces from all states between days 28 and 364, except for those from Ohio. Feral pig, waterfowl, and raccoon feces had populations of STEC of <1.0 log CFU/g starting from day 112 in feces from all states. E. coli O103 and O104 were the predominant serotypes throughout the entire storage period in feces from all animals and from all states. The survival of both O157 and non-O157 STEC strains in domesticated and wild animal feces indicates a potential risk of contamination from animal intrusion. HIGHLIGHTS

Publisher

International Association for Food Protection

Subject

Microbiology,Food Science

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3