Anticarcinogenic and antioxidant activities of leaves and flowers hydroalcoholic extracts of Nerium oleander L.: PCA analysis and phytochemical content by FTIR spectroscopy

Abstract

Aims: The aim of this work is the evaluation the anticarcinogenic effect on HT29 cancer cells lines and antioxidant effect of three extracts from different morphological parts (leaves (LE), pink flowers (PFE) and white flowers (WFE)) of Nerium oleander lin. Methods: This research provides the anticarcinogenic activity of extracts from N. oleander white flowers. Principal Component Analysis (PCA) was applied to estimate the relationship between antioxidant and anticarcinogenic activities, and to further understand the similarities and differences between the extracts. Results: All of the extracts exhibited different antioxidant (ABTS, DPPH and FC) and anticarcinogenic activities. FTIR analysis designed the existence of various functional groups in extracts confirming the presence of alcohol, alkanes, amino, aromatic compound and primary alcohol. The PCA results show that the WFE extract exhibits a greater antioxidant activity with a potent potential for inhibiting the cell growth of colorectal cancer HT29 than the two other extracts (LE and PFE). Conclusions: Nerium oleander may therefore be an excellent source of natural antioxidant and anticancer agents, as well as a possible pharmaceutical supplement. Keywords: Nerium oleander, antioxidant activity, anticarcinogenic activity, PCA analysis, FTIR analysis.