Isolation of protoplasts from viable sperm cells of common buckwheat (Fagopyrum esculentum Moench.)

Abstract

Protoplasts from sperm cells were isolated. Sperm cells from germinated pollen placed in solution were released, sieved and removed after sucrose density gradient centrifugation. The isolated cells were spherical and approximately 5.0–6.0 µm in diameter. Cytological observations confirmed that sperm cells of common buckwheat are true protoplasts. Key words: Common buckwheat (F. esculentum Moench.), sperm cell isolation, osmotic shock