Abstract
AbstractIn the present study, a high yield of isolated protoplasts from the agronomically important crop Fagopyrum esculentum was obtained by applying a mixture of cellulase, pectolyase, and driselase. We demonstrated that the yield of morphogenic callus-derived protoplasts was 1 × 106 protoplasts per g of fresh tissue. For hypocotyls used as the protoplast source, the number of released cells was twice lower. The protoplasts, embedded in an agarose matrix and cultured in a modified Kao and Michayluk media supplemented with phytosulfokine, re-enter the cell cycle and start to develop, forming microcalli. The plating efficiency was about 20% in the case of hypocotyl- and morphogenic callus-derived protoplasts. For plant regeneration, the medium was supplemented with different combinations of cytokinin. Somatic embryogenesis and organogenesis occur during the cultivation of the protoplast-derived tissues, depending on the applied protoplast source. For the first time, an effective protoplast-to-plant system for F. esculentum has been developed.
Publisher
Springer Science and Business Media LLC
Reference77 articles.
1. Adachi T (1990) How to combine the reproductive system with biotechnology in order to overcome the breeding barrier in buckwheat. Fagopyrum 10(1):7–11
2. Adachi T, Yamaguchi A, Miike Y, Hoffmann F (1989) Plant regeneration from protoplasts of common buckwheat (Fagopyrum esculentum). Plant Cell Rep 8(4):247–250. https://doi.org/10.1007/bf00778544
3. Adedeji OS, Naing AH, Kim CK (2020) Protoplast isolation and shoot regeneration from protoplast-derived calli of Chrysanthemum cv. White ND Plant Cell Tiss Org 141(3):571–581. https://doi.org/10.1007/s11240-020-01816-3
4. Bano AS, Khattak AM, Basit A, Alam M, Shah ST, Ahmad N, Gilani SAQ, Ullah I, Anwar S & Mohamed HI (2022) Callus induction, proliferation, enhanced secondary metabolites production and antioxidants activity of Salvia moorcroftiana L. as influenced by combinations of auxin, cytokinin and melatonin. Braz Arch Biol Techn 65
5. Berbec A, Doroszewska T (1999) Regeneration in vitro of three cultivars of buckwheat (Fagopyrum esculentum Moench.) as affected by medium composition. Fagopyrum 16:49–52
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献