EFFECT OF EQUILIBRATION TIME AT 5 °C AND PHOTOPERIOD ON SURVIVAL OF RAM SPERMATOZOA FROZEN IN STRAWS

Author:

FISER P. S.,BATRA T. R.

Abstract

The effect of various equilibration times at 5 °C on the cryosurvival of ram spermatozoa frozen in 0.5-mL straws was assessed for semen collected during decreasing and increasing photoperiods. The percentage and rate of forward progression of motile spermatozoa increased significantly from 0 to 2 h equilibration, remained constant from 2 to 6 h and decreased significantly at 18 h. Kinetic rating of motile spermatozoa changed similarly. Photoperiod has a significant (P < 0.01) effect on survival of spermatozoa at all equilibration times, but rate of forward progression was affected only at the 0 h equilibration time. Key words: Spermatozoa survival, equilibration time, frozen-thawed semen, rams

Publisher

Canadian Science Publishing

Subject

Animal Science and Zoology,Food Animals

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