Antibacterial resistance of modified woven vascular prostheses in experimental infected wound modeling

Abstract

Objective. To optimize the composition of polymer coatings based on the assessment of the duration of the antibacterial resistance in infected wounds of laboratory animals.Materials and methods. Woven vascular prostheses consisting of fibrous-porous polypropylene and one of the three types of coatings based on a polyvinyl-chitosan complex with the addition of biologically active substances were used in the study. All the samples were impregnated with 1% vancomycin for 30 minutes. The research methods were: bacteriological, molecular and genetic methods. Qualitative results of the antibacterial resistance of the coatings during the study period in the groups were confirmed with three or more repetitions of the results.Results. In the qualitative assessment of the duration of the antibacterial resistance of the prostheses according to the PCR and bacteriological studies, woven vascular prostheses with coatings containing polyvinyl alcohol, polyvinylpyrrolidone, chitosan, antibiotic vancomycin, and hyaluronic acid do not get infected with S. aureus for up to four days, and woven vascular prostheses with coatings consisting of polyvinyl alcohol, polyvinylpyrrolidone, chitosan, antibiotic vancomycin, and L-aspartic acid and woven prostheses with coatings of polyvinyl alcohol, chitosan, antibiotic vancomycin, and L–aspartic acid up to five days.Conclusion. The presence of L-aspartic acid in the composition of polymer coatings increases the duration of the antibacterial resistance of woven vascular prostheses in comparison with hyaluronic acid. The presence of polyvinylpyrrolidone in polymer coatings significantly (p = 0.012) reduces the contamination of S. aureus, increasing the antibacterial resistance of woven vascular prostheses, whereas polyvinyl alcohol does not have such an effect.