Prevalence of fluoroquinolone resistance-associated mutations inMycoplasma genitaliumamong clients of two sexual health centres in the Netherlands: a cross-sectional study

Abstract

ObjectiveThis study aimed to determine the prevalence of fluoroquinolone resistance-associated mutations (QRAMs) inMycoplasma genitalium(MG) among clients of two sexual health centres (SHCs) in the Netherlands.DesignA cross-sectional study.Setting and participantsBetween 2018 and 2019, 669 clients with MG were included from two previous studies: 375 male clients with urethritis from the SHC in Amsterdam; and 294 clients (male and female) from the SHC in Amsterdam and The Hague. Urogenital and anal samples (705 in total) that tested positive for MG by nucleic acid amplification tests were selected.Outcome measuresThe presence of QRAM was detected by an MG-QRAM PCR targeting four mutations in theparCgene and investigated by sequence analysis of relevant regions of thegyrAandparCgenes. Possible risk factors for the presence of QRAM were investigated.ResultsWe found QRAM in 58 of 669 (9%) clients with an MG infection: 36 of 375 (10%) in the study population of men with urethritis and 22 of 294 (7%) in the study population of other clients (including both men and women; p=0.334). Most prevalent mutations in theparCgene were S83I and D87N, occurring in 31 of 60 (52%) and 20 of 60 (33%) samples, respectively. Factors associated with the presence of QRAM were: men who have sex with men (adjusted OR (aOR) 3.4, 95% CI 1.7 to 6.9) and Asian origin (aOR 2.5, 95% CI 1.2 to 5.6). Multidrug resistance (QRAM plus macrolide resistance-associated mutations) was found in 46 of 669 (7%) clients.ConclusionsNine per cent of MG-positive clients from two Dutch SHCs had QRAM. New treatment strategies and antibiotics are needed to treat symptomatic patients with multidrug-resistant MG.