The effect of the combined use of tulatromycin and thymalin in genital mycoplasmosis in cows on cellular immunity indicators

Abstract

Modern technologies of industrial animal husbandry imply the most effective use of the reproductive potential of females and obtaining healthy young from them. The limiting factor in achieving this goal are diseases characterized by smoothed symptoms, which complicates their timely diagnosis and therapy, one of which is genital mycoplasmosis of cattle. The presence of a long latent period and unclear symptoms allows this disease to remain unnoticed and spread in the herd. During the latent period, morphofunctional changes develop in the body of females, leading to partial or complete loss of reproductive ability. Tetracycline, fluoroquinolone and macrolide antibiotics have been used with varying degrees of effectiveness for the treatment of cows with genital mycoplasmosis, however, antibiotic therapy alone does not restore all functions disrupted during the disease, and in particular does not eliminate immunodeficiency. In this regard, the purpose of our study was to study the inclusion of the immunomodulator thymalin in the treatment regimen of cows with genital mycoplasmosis and its effect on the content of leukocytes, lymphocytes and their subpopulations in the blood. Three groups of animals were formed for the experiment: the first group - pregnant cows with genital mycoplasmosis for the treatment of which the antibiotic traksovet 100 (tulatromycin) was used at a dose of 2.5 mg per 1 kg of animal body weight, subcutaneously, once 40 days before the expected delivery; the second group - pregnant cows with genital mycoplasmosis for the treatment of which the antibiotic traksovet 100 was used at the same dose and the immunomodulator timalin at a dose of 0.1 mg per 1 kg of animal body weight, intramuscularly, twice with an interval of 72 hours; the third group – clinically healthy pregnant cows. In all groups of cows, the number of leukocytes, the percentage of lymphocytes, the absolute and relative content of T-, B- and 0-lymphocytes were determined. It has been established that the use of tulatromycin for the treatment of cows with genital mycoplasmosis gives a good therapeutic effect, but provides only partial restoration of cellular immunity. The combined use of tulatromycin and thymalin has the maximum therapeutic effect and leads to the complete restoration of cellular immunity, and some indicators even slightly exceed their value in clinically healthy cows.