Cytotoxicity and apoptotic activity of magnesium orotate nanoparticles towards human hepatoma cell line HepG2

Abstract

The purpose of this study was to enhance the therapeutic effect of magnesium orotate) MO( via loading it to gum arabic nanocarrier. The magnesium orotate-loaded gum arabic nanoparticles) MOGANPs( were prepared by high pressure homogenizer. The freeze-dried powder of nanoparticles was characterized using X-ray Diffractometry )XRD( and Transmission Electron Microscopy )TEM(. The obtained nanoparticles were spherical in shape with diameters ranged from 30 to 50 nm. The cytotoxicity of MOGANPs was determined against human hepatocellular carcinoma HepG2 cell line using 3-)4,5-dimethylthiazol-2-yl (2,5-diphenyl tetrazolium bromide )MTT( assay which showed an enhanced efficacy of MO nano-carried with gum arabic when compared to pure compound alone. Moreover, the results revealed that MOGANPs induced apoptosis in the treated HepG2 cells and thus it is promising formulation, in addition, further anticancer studies are needed.