Streptomyces pratensis S10 Controls of Fusarium Head Blight by Suppressing Different Stages of the Life Cycle and ATP Production

Abstract

Fusarium head blight (FHB) of wheat, predominately caused by Fusarium graminearum, is an economically important plant disease worldwide. With increased fungicide resistance, controlling this filamentous fungal disease has become an enormous challenge. Biocontrol agents (BCAs) alone or integrated with other methods could better manage FHB. Streptomyces pratensis S10 has strong antagonistic activity against FHB as reported in our previous study. We now have investigated S10 controls of FHB in more detail by combining microscope observations, biological assays, and transcriptome profiling. S10 culture filtrates (SCF) significantly inhibited essential stages of the life cycle of F. graminearum in the laboratory and under simulated natural conditions. SCF at different concentrations inhibited conidiation of F. graminearum with an inhibition of 57.49-83.83% in the medium and 64.04-85.89% in plants. Different concentrations of SCF reduced conidia germination by 47.33 to 67.67%. 2% (vol/vol) SCF suppressed perithecia formation of F. graminearum by 84% and 81% in the laboratory and under simulated natural conditions, respectively. The S10 also reduced the pathogenicity and penetration ability of F. graminearum by suppressing ATP production. Collectively, these findings indicate that S. pratensis S10 should be explored further for efficacy at controlling FHB.