Ultrastructural Observations of Botrytis cinerea and Physical Changes in Resistant and Susceptible Grapevines

Author:

Hou Xiaoqing12,Zhang Guoyun3,Han Rui12,Wan Ran14,Li Zhi12ORCID,Wang Xiping12ORCID

Affiliation:

1. State Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Yangling 712100, Shaanxi, China

2. Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Northwest A&F University, Yangling 712100, Shaanxi, China

3. State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Yangling 712100, Shaanxi, China

4. College of Horticulture, Henan Agricultural University, Zhengzhou 450002, Henan, China

Abstract

The necrotrophic fungus Botrytis cinerea is a major threat to grapevine cultivation worldwide. Here, a highly resistant Chinese wild grapevine, Vitis amurensis ‘Shuangyou’ (SY), and the susceptible V. vinifera ‘Red Globe’ (RG) were selected for study, and their pathogenic infection and biochemical responses to B. cinerea were evaluated. The results revealed more trichomes on and a thicker cuticle for leaves of SY than RG under scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Both SEM and TEM also showed that conidial germination, appressorium formation, and hyphal development of B. cinerea were delayed on the leaves of resistant SY. Fewer infected hyphae were also observed in leaves of resistant SY when compared with susceptible RG. The infected leaves of resistant SY harbored higher levels of cellulase and pectinase activity during the early infection stages of B. cinerea at 4 h postinoculation (hpi), and higher glucanase and chitinase activity were maintained in the inoculated leaves of SY from 4 through 18 hpi. Lignin was deposited in the infected leaves of susceptible RG but not in resistant SY. Taken together, these results provide insights into the ultrastructural characterizations and physical changes in resistant and susceptible grapevines.

Funder

National Natural Science Foundation of China

Program for Innovative Research Team of Grape Germplasm Resources and Breeding

Publisher

Scientific Societies

Subject

Plant Science,Agronomy and Crop Science

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