Genome resource of Ancylobacter pratisalsi E130 T: A novel plant growth-promoting bacterium isolated from the rhizosphere

Abstract

Ancylobacter pratisalsi sp. nov. strain E130T is a Gram negative, non-motile, aerobic and rod-shaped bacterium, which was recently isolated from the rhizosphere of Plantago winteri Wirtg from a natural salt meadow. This strain was described as novel species in Ancylobacter genus, however information about its complete genome has yet not been reported. In this study, its genome was completely sequenced by PacBio SMRT cell platform, analyzed, and compared with other selected complete genome sequences of Ancylobacter and elucidated its potential plant growth promotion abilities. The genomic analysis revealed that the genome of strain E130T consists of one circular DNA chromosome of 4,618,530 bp with a GC content of 66% and one plasmid of 159,741 bp with a GC content of 64.13%. The entire genome contains 4,322 predicted coding genes, 49 tRNAs and 6 rRNA genes. Genome analysis identified a siderophore natural product biosynthesis cluster, which produces fuscachelin. Knockout of several key genes in this cluster, significantly reduces the plant growth-promotion ability of the strain E130T. Besides plant growth-promotion, the strain E130T can grow well on 5 % NaCl (w/v), conferring this strain as potential bio-resource for successful production of economic crops in alkaline soil.