<i>Ancylobacter crimeensis</i> sp. nov., a New Species of Aerobic Methylotrophic Bacteria Isolated from Oak Phyllosphere-Reference-Cited by-同舟云学术

<i>Ancylobacter crimeensis</i> sp. nov., a New Species of Aerobic Methylotrophic Bacteria Isolated from Oak Phyllosphere

Published:2023-09-01 Issue:5 Volume:92 Page:441-452
ISSN:0026-3656
Container-title:Микробиология
language:
Short-container-title:Microbiology

Author:

Belova A. A.¹,Kaparullina E. N.¹,Agafonova N. V.¹,Grouzdev D. S.²,Kopitsyn D. S.³,Machulin А. V.¹,Doronina N. V.¹

Affiliation:

1. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Federal Research Center “Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences”

2. SciBear OU

3. Gubkin University

Abstract

Abstract—A new facultative methylotroph, strain 6x-1T, was isolated from the phyllosphere of oak (Quercus pubescens Willd.) on the medium with methanol. Cells were aerobic, gram-negative, not-spore-forming, non-motile short ovoids rods reproducing by binary fission. Growth optimum was at 25‒29°C and pH 7.0‒7.5; growth was inhibited by 1.5% NaCl. Predominant fatty acids were C18:1ω7c and C19:0cyclo. Predominant phospholipids were phosphatidylethanolamine, phosphatidylmonomethylethanolamine, and diphosphatidylglycerol. Tha major ubiquinone was Q10. The 16S rRNA gene sequence of strain 6x-1T exhibited the highest similarity to those of members of the genus Ancylobacter (97.0‒97.4%). Genome analysis of strain 6x-1T and most closely related Ancylobacter strains revealed that the values of ANI (80.7‒83.5%), dDDH (22.4‒23.3%), AAI (72.0‒78.0%), and POCP (62.0‒69.0%) were below the recommended threshold values for prokaryotic species. Genome size of strain 6x-1T was 4.29 × 106 Mb, and G + C content was 67.3%. According to the results of phylogenetic, phylogenomic, phenotypic, and chemotaxonomic analysis, strain 6x-1T (=VKM В-3256T = ССUG 72401T) represents a new species of genus Ancylobacter, for which the name Ancylobacter crimeensis sp. nov. is proposed.

Publisher

The Russian Academy of Sciences

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