The Detriment of Salicylic Acid to the Pseudomonas savastanoi pv. phaseolicola Proteome

Abstract

Salicylic acid (SA), a natural product, is the major hormonal regulator of the plant immune system. SA also has antibacterial activity that is not completely elucidated. To gain a better understanding of this, Pseudomonas savastanoi pv. phaseolicola, a bacterial pathogen of beans, was exposed to sub-inhibitory amounts of SA and was then examined using quantitative mass spectrometry. Among the 2,185 proteins quantified, there were pronounced increases in p-hydroxybenzoic acid efflux pumps and multidrug efflux pumps. By contrast there were significant decreases in porin proteins, high-osmolarity response proteins, and protein components of the type VI secretion system. In addition, there were alterations in enzymes likely affecting the production of alginate, which is needed for infection. Furthermore, there was a decrease in an enzyme needed to detoxify methylglyoxal. Assays confirmed a reduction in alginate production and an increase in cellular methylglyoxal concentrations after SA treatment. Culture assays demonstrated that SA altered bacterial growth curves more so than other hydroxylated benzoic acid isomers. These data reveal that SA is antibiotic and that P. savastanoi pv. phaseolicola significantly alters its proteome in response to SA in vitro. Similar alterations to the bacterial proteome occur in beans during an immune reaction when SA increases at the site of infection. Thus, in beans, SA likely deters bacterial infection by adversely altering the bacterial proteome. [Formula: see text] The author(s) have dedicated the work to the public domain under the Creative Commons CC0 “No Rights Reserved” license by waiving all of his or her rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law, 2022.