High-Quality Complete Genome Sequence of Xanthomonas phaseoli pv. dieffenbachiae Outbreak Strain D182: The Causative Agent of Anthurium Bacterial Blight in Hawaii

Author:

Dobhal Shefali1,Chuang Shu-Cheng1,Arizala Dario1,Keith Lisa M.2,Alvarez Anne M.1,Arif Mohammad1ORCID

Affiliation:

1. Department of Plant and Environmental Protection Sciences, University of Hawaii at Manoa, Honolulu, HI

2. Tropical Plant Genetic Resources and Disease Research Unit, United States Department of Agriculture-Agricultural Research Service, Hilo, HI

Abstract

Xanthomonas phaseoli pv. dieffenbachiae (Xpd), the causal agent of anthurium blight, is classified as an A2 quarantine organism on the European and Mediterranean Plant Protection Organization (EPPO) list due to its devastating impact on the anthurium industry. In this study, we sequenced strain D182, representative of the Hawaiian anthurium blight outbreak (1981 to 1986), using PacBio RS II SMRT technology. High-quality de novo assembly of 5,217,888 bp (65% GC) was generated with a mean coverage of 351× using HGAP v4. Strain D182 harbors one plasmid (73.5 kb, 60.8% GC). Average nucleotide identity and digital DNA–DNA hybridization values of 99.86 and 98.9%, respectively, showed close phylogenetic relationships with Xpd strain LMG 695PT. The genome information will be useful in providing insights into the genomic biology, virulence mechanisms, and evolutionary relationships of Xpd and other strains associated with anthurium blight outbreaks worldwide. [Formula: see text] Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .

Funder

National Institute of Food and Agriculture

National Institute of General Medical Sciences

Publisher

Scientific Societies

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