Author:
Koui Tosséa A. Stéphane,Gnondjui Alloh Albert,Gbessi Adji Eric,Ako Ako Aristide Bérenger,Coulibaly Baba,Aka A. Delpêche,Gonedele Bi Sery E.,Toure Offiana André,Jambou Ronan
Abstract
Abstract
Background
Sickle cell disease (SCD) is a hemoglobin disorders that concern 300,000 newborns each year around the world. There are hemoglobin haplotypes that affect SCD clinic expression.
Methods
Our goal was to identify the hemoglobin’s haplotypes among individuals with mild malaria independently of SCD status in Côte d’Ivoire. To determine these haplotypes, specific restriction enzyme (RE) is used after PCR amplification with each primer. According to the digestion of PCR product by RE, five hemoglobin’s haplotypes are found in the world.
Results
In Côte d’Ivoire, no study has yet deeply described the distribution of haplotypes. Four different “classical” haplotypes of hemoglobin were detected: Benin (56.5%), Bantou (28.5%), Senegal (4%), Cameroun (1%); and 10% of atypical profiles. Heterozygous haplotype (69%) were more frequent than homozygous haplotype (31%).
Conclusions
In this preliminary study, we note a high prevalence of atypical and heterozygous haplotype. Benin haplotype that is associated with severity of SCD was most predominant in our studied population.
Publisher
Springer Science and Business Media LLC
Subject
Genetics (clinical),Genetics
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