Author:
Zhu Yufei,Zhao Jianfei,Wang Chenxu,Zhang Fei,Huang Xinhuo,Ren Zhouzheng,Yang Xin,Liu Yanli,Yang Xiaojun
Abstract
Abstract
Background
Many researches about in ovo feeding (IOF) of vitamin C (VC) are gradually carried out to explore physiological development in chicken, but little studies focus on VC synthesis capacity of the embryo itself, the selection of injection site and the effectiveness of IOF of VC. This study aims to explore the above problems.
Results
Kidney and yolk sac were the main organs for VC synthesis and L-gulonolactone oxidase (GLO) expression was lower during pre-hatch development than that during post-hatch development. Sodium-dependent vitamin C transporter 1 (SVCT1) expression was increased continuously in yolk sac from embryonic age 19 (E19) to post-hatch day 1 (D1) and in intestine (duodenum, jejunum and ileum) from E17 to D1. Plasma VC content was higher at D1 than that at D21 and D42. IOF of VC significantly reduced GLO expression in liver, kidney and yolk sac as well as SVCT1 expression in duodenum, jejunum and ileum, but increased the VC content in plasma, brain, kidney and liver. In addition, IOF of VC obviously reduced the embryonic morality and increased the hatchability under heat stress.
Conclusions
This study suggested that IOF of VC at E11 in yolk was effective for embryonic VC supplementation. These findings provide a theoretical reference about the method of embryonic VC supplementation and effective methodology on embryonic VC nutrition in broiler chickens.
Funder
the National Key Research and Development Program of China
the National Natural Science Foundation of China
Innovative Transforming Project of Shaanxi Agricultural Achievements in Science & Technology
the Program for Shaanxi Science & Technology from Shaanxi Provincial Science and Technology Department
Publisher
Springer Science and Business Media LLC
Subject
Animal Science and Zoology,Biochemistry,Food Science,Biotechnology
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