Abstract
Abstract
Background
Highly pathogenic influenza A (H5N8) viruses have caused several worldwide outbreaks in birds and are of potential risk to humans. Thus, a specific, rapid and sensitive method for detection is urgently needed.
Methods
In the present study, TaqMan minor groove binder probes and multiplex real-time RT-PCR primers were designed to target the H5 hemagglutinin and N8 neuraminidase genes. A total of 38 strains of avian influenza viruses and other viruses were selected to test the performance of the assay.
Results
The results showed that only H5 and N8 avian influenza viruses yielded a positive signal, while all other subtypes avian influenza viruses and other viruses were negative. High specificity, repeatability, and sensitivity were achieved, with a detection limit of 10 copies per reaction.
Conclusions
The developed assay could be a powerful tool for rapid detection of H5N8 influenza viruses in the future.
Funder
the National Science and Technology Major Project for the Control and Prevention of Major Infectious Diseases in China
Zhejiang Provincial Natural Science Foundation of China
Publisher
Springer Science and Business Media LLC
Cited by
8 articles.
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