A Paper-based Loop-Mediated Isothermal Amplification (LAMP) Assay for Highly Pathogenic Avian Influenza-Reference-Cited by-同舟云学术

A Paper-based Loop-Mediated Isothermal Amplification (LAMP) Assay for Highly Pathogenic Avian Influenza

Published:2024-08-12 Issue: Volume: Page:
ISSN:
Container-title:
language:
Short-container-title:

Author:

Kamel Mohamed,Davidson Josiah Levi,Verma Mohit S.^ORCID

Abstract

AbstractAvian influenza outbreaks have had significant economic and public health consequences worldwide. Therefore, prompt, reliable, and cost-effective diagnostic devices are crucial for scrutinizing and confining highly pathogenic avian influenza viruses (HPAIVs). Our study introduced and evaluated a novel paper-based loop-mediated isothermal amplification (LAMP) test for diagnosing the H5 subtype of the avian influenza virus (AIV). We meticulously designed and screened LAMP primers targeting the H5-haemagglutinin (H5-HA) gene of AIV and fine-tuned the paper-based detection assay for best performance. The paper-based LAMP assay demonstrated a detection limit of 500 copies per reaction (25 copies/µL). This inexpensive, user-friendly point-of-need diagnostic tool holds great promise, especially in resource-limited settings. It only requires a water bath for incubation and enables visual detection of results without special equipment. Overall, the paper-based LAMP assay provides a promising method for rapidly and reliably detecting the H5 subtype of AIV, contributing to improved surveillance and early intervention strategies.

Publisher

Cold Spring Harbor Laboratory

Reference69 articles.

1. Badara, O. Wildlife under threat as avian influenza reaches Antarctica. WOAH - World Organisation for Animal Health https://www.woah.org/en/wildlife-under-threat-as-avian-influenza-reaches-antarctica/ (2024).

2. USDA, A. USDA APHIS | USDA Confirms Highly Pathogenic Avian Influenza in a Commercial Turkey Flock in Dubois County, Indiana. (2023).

3. The resurgence of Avian influenza and human infection: A brief outlook;New Microbes New Infect,2023

4. APHIS. Highly Pathogenic Avian Influenza (HPAI) Detections in Livestock | Animal and Plant Health Inspection Service. https://www.aphis.usda.gov/livestock-poultry-disease/avian/avian-influenza/hpai-detections/livestock (2024).

5. AVMA. Avian influenza virus type A (H5N1) in U.S. dairy cattle | American Veterinary Medical Association. https://www.avma.org/resources-tools/animal-health-and-welfare/animal-health/avian-influenza/avian-influenza-virus-type-h5n1-us-dairy-cattle (2024).