Author:
Seidl Verena,Gamauf Christian,Druzhinina Irina S,Seiboth Bernhard,Hartl Lukas,Kubicek Christian P
Abstract
Abstract
Background
The hypercellulolytic mutant Hypocrea jecorina (anamorph Trichoderma reesei) RUT C30 is the H. jecorina strain most frequently used for cellulase fermentations and has also often been employed for basic research on cellulase regulation. This strain has been reported to contain a truncated carbon catabolite repressor gene cre1 and is consequently carbon catabolite derepressed. To date this and an additional frame-shift mutation in the glycoprotein-processing β-glucosidase II encoding gene are the only known genetic differences in strain RUT C30.
Results
In the present paper we show that H. jecorina RUT C30 lacks an 85 kb genomic fragment, and consequently misses additional 29 genes comprising transcription factors, enzymes of the primary metabolism and transport proteins. This loss is already present in the ancestor of RUT C30 – NG 14 – and seems to have occurred in a palindromic AT-rich repeat (PATRR) typically inducing chromosomal translocations, and is not linked to the cre1 locus. The mutation of the cre1 locus has specifically occurred in RUT C30. Some of the genes that are lacking in RUT C30 could be correlated with pronounced alterations in its phenotype, such as poor growth on α-linked oligo- and polyglucosides (loss of maltose permease), or disturbance of osmotic homeostasis.
Conclusion
Our data place a general caveat on the use of H. jecorina RUT C30 for further basic research.
Publisher
Springer Science and Business Media LLC
Reference54 articles.
1. Fierro F, Garcia-Estrada C, Castillo NI, Rodriguez R, Velasco-Conde T, Martin JF: Transcriptional and bioinformatic analysis of the 56.8 kb DNA region amplified in tandem repeats containing the penicillin gene cluster in Penicillium chrysogenum. Fungal Genet Biol. 2006, 43: 618-629. 10.1016/j.fgb.2006.03.001.
2. Keller NP, Turner G, Bennett JW: Fungal secondary metabolism – from biochemistry to genomics. Nature Rev Microbiol. 2005, 3: 937-947. 10.1038/nrmicro1286.
3. Rodriguez-Saiz M, Barredo JL, Moreno MA, Fernandez-Canon JM, Penalva MA, Diez B: Reduced function of a phenylacetate-oxidizing cytochrome p450 caused strong genetic improvement in early phylogeny of penicillin-producing strains. J Bacteriol. 2001, 183: 5465-5471. 10.1128/JB.183.19.5465-5471.2001.
4. Reese ET, Mandels M: Rolling with the times: production and applications of Trichoderma reesei cellulases. Annu Rep Ferm Proc. 1984, 7: 1-20.
5. El-Gogary S, Leite A, Crivellaro O, El-Dorry H, Eveleigh DE: Trichoderma reesei cellulase – from mutants to induction. Trichoderma reesei cellulases. Edited by: Kubicek CP, Eveleigh DE, Esterbauer H, Steiner W, Kubicek-Pranz EM. 1990, Royal Society of Chemistry, Cambridge, UK, 200-211.
Cited by
135 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献