Screening, Identification, and Optimization of Enzyme-Producing Conditions for Cellulose-Degrading Bacteria in Distillery Lees
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Published:2023-06-29
Issue:13
Volume:13
Page:7693
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ISSN:2076-3417
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Container-title:Applied Sciences
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language:en
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Short-container-title:Applied Sciences
Author:
Luo Ai-Guo12,
Wang Yuan-Yuan3,
Xue Sha-Sha3,
Zhao Jia12,
Hao Jian-Wei12,
Shi Sheng-Li12,
Hu Bian-Fang12
Affiliation:
1. Department of Biological Science and Technology, Jinzhong University, Jinzhong 030619, China
2. Shanxi Higher School Solid Fermentation Engineering Technology Research Center, Jinzhong University, Jinzhong 030619, China
3. Food Science and Engineering, Shanxi Agricultural University, Jinzhong 030801, China
Abstract
Cellulose-degrading bacteria were identified from distillery lees, and the strains were optimized for fermentation and enzyme production, providing effective strains for the resource utilization of distillery lees and developing cellulase. Based on the univariate test, the response surface test was used to optimize enzyme production conditions for fermentation. The screened strain JZ2 had a clear circle-to-colony diameter ratio of 2.0. The enzyme activities of exoglucanase, endoglucanase, and β-glucosidase were 4.341 ± 0.05 U/mL, 1.874 ± 0.04 U/mL, and 0.739 ± 0.02 U/mL, respectively. The bacterial colonies were large, and the bacterial cells’ morphology was rod-shaped. We identified Bacillus cereus (JZ2) from 16S rDNA sequence homology and phylogenetic tree analysis as belonging to the genus Bacillus in the thick-walled Bacillus phylum. The optimum production conditions of cellulase from strain JZ2 were a fermentation time of 2 d, a rotation rate of 180× g, and a temperature of 26 °C using the response surface method. The enzyme activity of JZ2 was 4.625 U/mL under optimal enzyme production conditions. In addition to good activity, the cellulase from the JZ2 strain may have the potential to convert distillery lees cellulose into useful compounds.
Funder
Shanxi Scholarship Council of China
Shanxi Province Scholarship Program for Scientific and Technological Activities of Overseas Students
Shanxi Provincial Higher Education Solid State Brewing Engineering Technology Research Center
Collaborative innovation center for high-value utilization of brewing by-product resources
Subject
Fluid Flow and Transfer Processes,Computer Science Applications,Process Chemistry and Technology,General Engineering,Instrumentation,General Materials Science
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