Applicability of RNA standards for evaluating RT-qPCR assays and platforms-Reference-Cited by-同舟云学术

Applicability of RNA standards for evaluating RT-qPCR assays and platforms

Published:2011-02-18 Issue:1 Volume:12 Page:
ISSN:1471-2164
Container-title:BMC Genomics
language:en
Short-container-title:BMC Genomics

Author:

Devonshire Alison S,Elaswarapu Ramnath,Foy Carole A

Abstract

Abstract The availability of diverse RT-qPCR assay formats and technologies hinder comparability of data between platforms. Reference standards to facilitate platform evaluation and comparability are needed. We have explored using universal RNA standards for comparing the performance of a novel qPCR platform (Fluidigm® BioMark™) against the widely used ABI 7900HT system. Our results show that such standards may form part of a toolkit to evaluate the key performance characteristics of platforms.

Publisher

Springer Science and Business Media LLC

Subject

Genetics,Biotechnology

Link

https://link.springer.com/content/pdf/10.1186/1471-2164-12-118.pdf

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