Development and Analytical Validation of a One-Step Five-Plex RT-ddPCR Assay for the Quantification of SARS-CoV-2 Transcripts in Clinical Samples

Author:

Strati Areti1ORCID,Zavridou Martha1,Paraskevis Dimitrios2,Magiorkinis Gkikas2,Sapounas Spyros3,Lagiou Pagona2,Thomaidis Nikolaos S.1ORCID,Lianidou Evi S.1ORCID

Affiliation:

1. Lab of Analytical Chemistry, Department of Chemistry, National and Kapodistrian University of Athens, 15771 Athens, Greece

2. Department of Hygiene, Epidemiology and Medical Statistics, Medical School, National and Kapodistrian University of Athens, 11527 Athens, Greece

3. National Public Health Organization, 15123 Athens, Greece

Funder

National and Kapodistrian University of Athens

Publisher

American Chemical Society (ACS)

Subject

Analytical Chemistry

Reference40 articles.

1. Molecular Diagnosis of a Novel Coronavirus (2019-nCoV) Causing an Outbreak of Pneumonia

2. Diagnostics for SARS-CoV-2 infections

3. Diagnostic testing and screening for SARS-CoV-2 https://www.ecdc.europa.eu/en/covid-19/latest-evidence/diagnostic-testing (accessed on Oct 4, 2021).

4. CDC Diagnostic Tests for COVID-19 | CDC https://www.cdc.gov/coronavirus/2019-ncov/lab/testing.html (accessed on Oct 4, 2021).

5. Test for Past Infection|CDC https://www.cdc.gov/coronavirus/2019-ncov/testing/serology-overview.html (accessed on Oct 4, 2021).

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