Author:
Li Yige,Hu Yangnan,Wei Hao,Cao Wei,Qi Yanru,Zhou Shan,Zhang Panpan,Li Huawei,Li Geng-Lin,Chai Renjie
Abstract
Abstract
Background
The ideal neural interface or scaffold for stem cell therapy shall have good biocompatibility promoting survival, maturation and integration of neural stem cells (NSCs) in targeted brain regions. The unique electrical, hydrophilic and surface-modifiable properties of Ti3C2Tx MXene make it an attractive substrate, but little is known about how it interacts with NSCs during development and maturation.
Results
In this study, we cultured NSCs on Ti3C2Tx MXene and examined its effects on morphological and electrophysiological properties of NSC-derived neurons. With a combination of immunostaining and patch-clamp recording, we found that Ti3C2Tx MXene promotes NSCs differentiation and neurite growth, increases voltage-gated current of Ca2+ but not Na+ or K+ in matured neurons, boosts their spiking without changing their passive membrane properties, and enhances synaptic transmission between them.
Conclusions
These results expand our understanding of interaction between Ti3C2Tx MXene and NSCs and provide a critical line of evidence for using Ti3C2Tx MXene in neural interface or scaffold in stem cell therapy.
Funder
Postgraduate Research & Practice Innovation Program of Jiangsu Province
National Natural Science Foundation of China
National Key R&D Program of China
Strategic Priority Research Program of the Chinese Academy of Science
Natural Science Foundation from Jiangsu Province
Shenzhen Fundamental Research Program
Open Research Fund of State Key Laboratory of Genetic Engineering, Fudan University
Science and Technology Department of Sichuan Province
Publisher
Springer Science and Business Media LLC
Subject
Pharmaceutical Science,Applied Microbiology and Biotechnology,Biomedical Engineering,Molecular Medicine,Medicine (miscellaneous),Bioengineering
Cited by
17 articles.
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