Protective role of small extracellular vesicles derived from HUVECs treated with AGEs in diabetic vascular calcification
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Published:2022-07-16
Issue:1
Volume:20
Page:
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ISSN:1477-3155
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Container-title:Journal of Nanobiotechnology
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language:en
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Short-container-title:J Nanobiotechnol
Author:
Guo Bei, Shan Su-Kang, Xu Feng, Lin Xiao, Li Fu-Xing-zi, Wang Yi, Xu Qiu-Shuang, Zheng Ming-Hui, Lei Li-Min, Li Chang-Chun, Zhou Zhi-Ang, Ullah Muhammad Hasnain Ehsan, Wu Feng, Liao Xiao-Bo, Yuan Ling-QingORCID
Abstract
AbstractThe pathogenesis of vascular calcification in diabetic patients remains elusive. As an effective information transmitter, small extracellular vesicles (sEVs) carry abundant microRNAs (miRNAs) that regulate the physiological and pathological states of recipient cells. In the present study, significant up-regulation of miR-126-5p was observed in sEVs isolated from human umbilical vein endothelial cells (HUVECs) stimulated with advanced glycation end-products (A-EC/sEVs). Intriguingly, these sEVs suppressed the osteogenic differentiation of vascular smooth muscle cells (VSMCs) by targeting BMPR1B, which encodes the receptor for BMP, thereby blocking the smad1/5/9 signalling pathway. In addition, knocking down miR-126-5p in HUVECs significantly diminished the anti-calcification effect of A-EC/sEVs in a mouse model of type 2 diabetes. Overall, miR-126-5p is highly enriched in sEVs derived from AGEs stimulated HUVECs and can target BMPR1B to negatively regulate the trans-differentiation of VSMCs both in vitro and in vivo.
Graphical Abstract
Funder
National Natural Science Foundation of China Fundamental Research Funds for Central Universities of the Central South University Key R&D Plan of Hunan Province Natural Science Foundation of Hunan Province
Publisher
Springer Science and Business Media LLC
Subject
Pharmaceutical Science,Applied Microbiology and Biotechnology,Biomedical Engineering,Molecular Medicine,Medicine (miscellaneous),Bioengineering
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